李中华
李中华，青年副研究员
联系方式
电子邮箱：zhonghua_@fudan.edu.cn
办公地点：科研二号楼A4-028
研究方向
方向一： 利用基于细胞工厂的糖链制备方法和糖芯片技术来进行人源糖链库的制备和功能糖组学的研究；
方向二：利用酶化学联合方法富集、标记、鉴定并定量疾病相关的O-糖修饰,比如单糖O-GlcNAc修饰和二糖TF(Core 1)修饰。
学术任职
美国糖生物学协会会员
中国生物物理学会糖生物学分会会员
学术报告
1.2021年全国糖科学青年论坛：
线上报告（广西南宁）2021年11月6-7日
基于于胞工厂的 O-GalNAc 糖组库制备及其功能糖组学研究
2.NIH Common Fund Glycoscience Annual Meeting：
Natcher Conference Center, Bethesda, MD2019年5月29-30日
Novel Technologies for Quantitative O-Glycomics and Amplification/Preparation of Cellular O-Glycans
3.Glycomics Data Club：
Emory Comprehensive Glycomics Core, Atlanta, GA2018年11月13日
A Strategy for Amplifying Cellular Glycans
4.NIH Common Fund Glycoscience Annual Meeting：
Natcher Conference Center, Bethesda, MD2018年6月2-3日
Novel Technologies for Quantitative O-Glycomics and Amplification/Preparation of Cellular O-Glycans
重要学术贡献
1）利用活细胞作为O-GalNac多糖的扩增工厂，发明了pCORA技术，建立了目前已知的糖链结构最丰富的人源O-GalNAc糖组库和O-糖芯片，实现了对活细胞O-GalNAc糖组的高灵敏度分析（比传统方法灵敏100-1000倍）和快速制备；
2）利用酶化学方法建立了高灵敏度和高选择性标记肿瘤相关的TF O-GalNAc二糖和O-GlcNAc单糖的方法，实现了对蛋白上O-糖链修饰集富集、定量、位点鉴定和可视化等多重优势于一体的检测。
荣誉与获奖
获上海市人才揽蓄行动项目 2022 年
发表论文
1. M. R. Kudelka#, Z. Li#, T. A. Chernova, D. F. Smith, X. Song, R. D. Cummings, T. Ju. Cellular O-glycome reporter/amplification (CORA): analytical and preparative tools to study mucin-type O-glycans of living cells. Current Protocols. 2021 Jun;1(6): e142. doi: 10.1002/cpz1.142. (共一)
2. Z. Li, Q. Zhang, D. Ashline, Y. Zhu, Y. Lasanajak, T. A. Chernova, V. Reinhold, R. D. Cummings, P. G. Wang, T. Ju, D. F. Smith, X. Song. Amplification and Preparation of Cellular O-glycome for Functional O-Glycomics. Analytical Chemistry, 2020, 92(15): 10390-10401.
3. Q. Zhang#, Z. Li#, T. A. Chernova, V. Saikam, R.D. Cummings, X. Song, T. Ju, D. F. Smith, P. G. Wang. Synthesis and Characterization of Versatile O-glycan Precursors for Cellular O-glycomics. ACS Synthetic Biology. 2019, 11, 2507-2513. (共一)
4. J. Li#, Z. Li#, X. Duan, K. Qin, L. Dang, S. Sun, L. Cai, L. C. Hsieh-Wilson, L. Wu, W. Yi. An Isotope-Coded Photocleavable Probe for Quantitative Profiling of Protein O-GlcNAcylation. ACS Chemical Biology. 2019,14,1,4-10. (共一)
5. Q. Li#, Z. Li#, X. Duan, W. Yi. A Tandem Enzymatic Approach for Detecting and Imaging Tumor-Associated Thomsen-Friedenreich Antigen Disaccharide. Journal of the American Chemical Society. 2014, 136, 12536-9. (共一)
6. Z. Li, W. Yi. Regulation of cancer metabolism by O-GlcNAcylation. Glycoconjugate Journal. 2014, 31, 185-91.
7. Z. Li, T. Li, S. Dai, X. Xie, X. Ma, W. Zhao, W. Zhang, J. Li, P. G. Wang. New Insights into the Pharmacological Chaperone Activity of C2-substituted Glucoimidazoles for the Treatment of Gaucher Disease. Chembiochem. 2013, 14, 1239-1247.
8. Z. Li, X. Zhang, X. Han, C. Feng, F. Wang, P. G. Wang, J. Shen, Y. Shi. Antitumor effects of a novel histone deacetylase inhibitor NK-HDAC-1 in breast cancer. Oncology Reports. 2013, 30, 499-505.
9. J. Hou#, Z. Li#, Q. Fang, C. Feng, H. Zhang, W. Guo, H. Wang, G. Gu, Y. Tian, P. Liu, R. Liu, J. Lin, Y. Shi, Z. Yin, J. Shen, P. G. Wang. Discovery and Extensive in Vitro Evaluations of NK-HDAC-1: A Chiral Histone Deacetylase Inhibitor as a Promising Lead. Journal of Medicinal Chemistry. 2012, 55, 3066. (共一)
10. T. Li#, Z. Li#, J. Li, J. Wang, L. Guo, P. G. Wang, W. Zhao. Elevation of cellular O-GlcNAcylation level by a potent and selective O-GlcNAcase inhibitor based on tetrahydroimidazopyridine scaffold. Bioorganic & Medicinal Chemistry Letters. 2012, 22, 6854. (共一)
专著
Z. Li, T. A. Chernova, T. Ju. Novel Technologies for Quantitative O-Glycomics and Amplification/Preparation of Cellular O-Glycans. Synthetic Glycomes. 2019, chap 16, 370-392.
专利
P. G. wang, J. Shen, Z. Yin, J. Hou, Z. Li, et al. Histone deacetylase inhibitors with branched structure synthesized through click chemistry. WO2011003365